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KMID : 0374019790020040195
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Ewha Medical Journal
1979 Volume.2 No. 4 p.195 ~ p.199
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Effects of Thiamine Deficiency on Drug Hydroxylation and Level of Cytochrome P-450 in the Rats
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Abstract
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Our studies are concerned with cytochrome P-450 content and 2-acetylaminofluorene N-and ring-hydroxylation by thiamine deficient rat liver microsomes.
Incubation medium contained 100mM HEPES buffer pH7.8, 2mM HADPH, 100mM KF, 100nM [^(14)C]-AAF and microsomal protein, After 30min, incubtion at 37°C, ring and N-hydroxy-9-^(14)C-AAF formations are assayed by radioactivity measurements after paper chromatotraphy sepation.
The concentration of cytochrome P-450 is elevated in microsomes from rats fed the deficient diet. It appears that the thiamine deficient rat liver is capable of producing effects on on the drug hydroxylation enzymes.
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